FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004920119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7708059
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT523700.679418774557901No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA296070.3841044807778456No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT243550.3159680018017506No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG232490.30161938303793473No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT211470.2743492233258723No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT206600.268031160633306No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166160.21556659075910029No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT143360.1859871596727529No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG124900.16203819924056106No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117340.15223028261719324No Hit
TTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATT100360.1302013905186766No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA94320.12236543596773196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89330.11589169205892172No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT85000.11027419483945311No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA81990.10636919099866776No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT81970.10634324412929377No Hit
ATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTA79440.10306096515348417No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA79320.10290528393724022No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77930.10110197651574801No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC77640.10072574690982516No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)