FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3801331
Sequences flagged as poor quality0
Sequence length125
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316700.8331292381536888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124910.3285954314423027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97130.25551576539901416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA253650.073.847551
GGTATCA90800.067.30311
TATCAAC349100.053.7841152
GTTATCA29100.052.597381
ATCAACG357900.052.4133033
CAACGCA363100.051.7553255
TCAACGC363600.051.63794
AACGCAG369500.050.813376
ACGCAGA388050.048.355447
CGCAGAG393850.047.6433378
GCAGAGT405350.045.461829
TTATCAA43550.035.0162352
CAGAGTA401400.032.55683510-11
AGAGTAC394700.031.74590310-11
GAGTACT177950.031.17418312-13
GTACTTT188900.029.41162114-15
AGTACTT180550.029.06401812-13
GAGTACG259550.028.87936412-13
AGTACGG259200.028.12889312-13
GTACGGG274500.026.72444314-15
Produced by FastQC (version 0.11.5)