FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004920147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740167
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28730.3881556459555749No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACAACCCCCCGTACTCTGCGTT18040.2437287801266471No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17830.2408915825752837No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT17640.23832459431452632No Hit
TGAAATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGC17370.2346767688913448No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC16470.22251735081407303No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACAACCCCACGTACTGCAGTCA16080.21724826964725527No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT15800.21346533957877076No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG14930.20171123543740807No Hit
TCATGTAACAAGCAAAATGAAACCACCAGAAACCAGGAACGTACTGCAGT12900.1742849924408951No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12560.16969143450059243No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT10750.1452374937007459No Hit
TCATAAAAAGGTCAATCCAACCACCCAAATCAAGCGTTACGTACTGCAGT9420.12726857587544432No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC8990.12145907612741448No Hit
TCAGGTAACAAGCTAAATGAAACCACCAGAAACCAGGAACGTACTGCAGT8790.1187569832213541No Hit
TCATAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGC8770.11848677393074805No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT8700.11754104141362694No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT8030.10848903017832462No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACCTGTCTCTTATACACATCTC7520.10159869326787063No Hit
TGAAATACAGAGAAAAAGGCCTGTCTCTTATACACATCTCCGAGCCCACG7470.10092317004135552No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)