FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4951914
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160920.3249652558586438No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127070.2566078490054553No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC98890.19970056022782304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94650.19113821443587267No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA85360.17237779169832107No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC80580.16272495847060348No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC68970.1392794786016074No Hit
AGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66560.13441267356420164No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA65850.13297888452828543No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC62140.1254868319603289No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59000.11914584946345998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG29750.077.388271
ATATCAA152000.077.134011
ATACGGG47000.073.1797261
AGTATCA61750.064.771131
TATCAAC362150.051.9567262
ATCAACG375750.050.209353
CAACGCA388950.048.7209055
TCAACGC393250.048.1311844
AACGCAG398550.047.8003356
ACGCAGA414550.045.739237
CGCAGAG421750.044.998238
GCAGAGT434600.043.3970839
GTATCAA169100.042.0376321
ATATACG6250.037.6434631
GGTATCA75900.037.27091
AGAGTAC435400.030.85090310-11
ACCTAGT8050.029.9221531
CAGAGTA441500.029.18761610-11
GAGTACT172350.027.70704512-13
AGTACGG320300.026.83595712-13
Produced by FastQC (version 0.11.5)