FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4951914
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA114960.23215265854778575No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC81060.16369428063572994No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC72670.14675133695779047No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA69750.14085462711993785No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC66730.13475597516435062No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC64130.12950548010324897No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT59980.12112488221725982No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC55140.11135088371890141No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA188300.065.9566651
GGTATCA89700.054.3347931
TATCAAC262150.047.150512
ATCAACG263250.046.682213
CAACGCA271650.045.17485
ACGCAGA274550.044.564877
AACGCAG277700.044.4263576
TCAACGC279100.044.201724
CGCAGAG284600.042.991168
GCAGAGT293800.041.31719
AGAGTAC287250.032.27445210-11
CAGAGTA292600.028.8880610-11
ATTTAGG70750.027.6729851
AGTACGG334000.027.42463312-13
TTTAGGG75150.026.7662432
GGGCTAT69800.026.6021336
GCTATTG70600.026.467688
CTATTGA72450.025.6252259
GGCTATT73700.025.515887
TATTCTC44800.025.5058925
Produced by FastQC (version 0.11.5)