FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6445228
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA194620.30195983757285233No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC135390.21006239034522906No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC109850.17043617386382606No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC97020.15052997349356764No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA94260.14624773553394851No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC93030.1443393468780313No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT81860.12700869542551482No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC71470.1108882416572385No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA71200.11046932707423228No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC66000.10240134251263106No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA248900.067.559051
GGTATCA115900.053.136531
TATCAAC352250.047.6351622
ATCAACG354950.046.9200553
CAACGCA362900.045.9609345
AACGCAG369400.045.4022836
ACGCAGA365900.045.1756677
TCAACGC375750.044.4005434
CGCAGAG378600.043.642868
GCAGAGT394000.041.7293249
AGAGTAC380000.033.67632710-11
CAGAGTA390650.028.80806710-11
AGTACGG448500.028.35909812-13
GCTATTG89900.026.322598
GGGCTAT89900.026.3205456
ATTTAGG94100.025.5463051
TTTAGGG95250.025.1750072
AGGGCTA94650.025.1383725
GGCTATT95700.024.7903777
CTATTGA96550.024.6348619
Produced by FastQC (version 0.11.5)