FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4711831
Sequences flagged as poor quality0
Sequence length150
%GC37

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81220.17237460341850122No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67280.14278950157592665No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT54080.11477491446531082No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA41300.043.4066735
AACGCAG41100.043.2675556
TCAACGC42300.042.8902134
CGCAGAG41200.042.6382988
ACGCAGA44150.039.7893037
ATCAACG46750.039.269613
GGGGTTA30800.035.7641536
GTATCAA59100.034.8281971
GAGTACG18500.034.744971
TAGGGGT31150.033.512594
GGGTTAG33050.032.89377
GTACGGG23300.032.2367321
GCAGAGT58150.030.952639
ATAGGGG33150.030.839183
AGTACGG21750.029.789792
AGGGGTT34450.029.2580975
TCGGACC2502.6429916E-928.7977144
GTACTTT80100.028.7628421
GTGGTAT46050.028.5440351
TACGGGC4050.026.66372
Produced by FastQC (version 0.11.5)