FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4532518
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA189650.064.634931
GGTATCA92950.048.205711
ATCAACG259250.047.007693
CAACGCA260600.046.903255
TATCAAC261650.046.689712
TCAACGC262050.046.464
AACGCAG268850.045.597276
ACGCAGA273300.044.570797
CGCAGAG284750.042.9034848
GCAGAGT296750.040.9457059
AGAGTAC279550.032.60794410-11
CAGAGTA288500.028.65740810-11
AGTACGG325450.027.48634312-13
GAGTACG327450.024.84735512-13
TACGGGT62000.024.13442214-15
TACGGGG103650.024.07982314-15
TACGGGC87600.023.66951214-15
GTACGGG333500.023.66475514-15
TACGGGA125050.021.67181614-15
GAAAAAA152700.017.847581
Produced by FastQC (version 0.11.5)