FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6371451
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT481480.7556834385134563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211960.3326714746766474No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165990.26052150444223776No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA376400.075.212721
GGTATCA145050.071.408321
TATCAAC504250.056.009332
ATCAACG514900.055.1162833
TCAACGC520450.054.802114
CAACGCA530850.053.8064045
AACGCAG533300.053.6140866
ACGCAGA565750.050.527627
CGCAGAG583350.049.0431488
GCAGAGT615900.046.5284169
GCGTAAG9200.040.6932031
AGAGTAC600950.033.84879310-11
CAGAGTA611500.031.82675710-11
AGTACGG450900.027.96252412-13
GAGTACT277300.027.67296812-13
AGTACTT291200.026.40305112-13
GAGTACG453450.025.944612-13
GTACTTT301600.025.51753214-15
TACGGGT94350.024.33218614-15
GTACGGG479250.023.64735614-15
Produced by FastQC (version 0.11.5)