FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6371451
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA242550.073.104521
GGTATCA102000.057.69331
TATCAAC347400.050.745832
ATCAACG348400.050.3936583
TCAACGC350150.050.1406144
AACGCAG357400.049.1123166
CAACGCA358500.048.8836365
ACGCAGA365500.048.1109357
CGCAGAG379350.046.4152958
GCAGAGT402800.043.7017829
AGAGTAC387650.034.24192410-11
AGTACGG449750.029.36753512-13
CAGAGTA399600.029.32612810-11
GAGTACG453600.025.70300712-13
TACGGGC116550.024.54678514-15
TACGGGT106150.024.17812514-15
TACGGGA175250.023.85937914-15
GTACGGG480200.023.52147914-15
TACGGGG145200.022.42742214-15
GTATTAG29350.019.259491
Produced by FastQC (version 0.11.5)