FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004920248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2849816
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA84850.29773852066238665Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT75970.26657861419824996Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT40800.14316713780819534No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG35120.1232360264662701Clontech Universal Primer Mix Long (96% over 26bp)
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGC32900.11544604985023593No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG32370.11358628065811968Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAAGAGTGTCGTATATCTGTCTCTTATA31620.1109545318013514No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT29150.10228730556639447No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)