FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004920251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2751954
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273750.9947477319751711No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175600.6380920611318358No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166570.6052790126579151No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT44880.16308412131888833Clontech Universal Primer Mix Long (96% over 26bp)
TTCTCATTCTTCCTTCCTGTCTTGCTACCCTCACAGCCTCATCCAAAAGA39850.1448061995222304No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA38180.13873778413447316Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT37050.13463161084814645No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36740.13350513853065857No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT36740.13350513853065857No Hit
GTATCAACGCAGAGTACATGGGAAAGAGTGTCGTATATCTGTCTCTTATA30070.1092678147963229No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29130.10585206002716614Clontech SMART CDS Primer II A (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)