FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004920252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2751954
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA46980.17071506282445129Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT43100.1566159899475064Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT39500.1435343759379699No Hit
TTCTCATTCTTCCTTCCTGTCTTGCTACCCTCACAGCCTCATCCAAAAGA36240.13168824769600074No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGC30340.11024893584703813No Hit
ATATACGACACTCTTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA30170.10963119296325448No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT27860.10123715730713523No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)