FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5512372
Sequences flagged as poor quality0
Sequence length125
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285150.5172909230363988No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96890.1757682536664797No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84640.15354551543328354No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA288750.075.7996751
GGTATCA107450.057.408611
TATCAAC396900.055.2441672
ATCAACG411050.053.2737433
TCAACGC412800.053.22454
CAACGCA414500.053.0467955
AACGCAG430300.051.138096
ACGCAGA451800.048.704567
CGCAGAG460600.047.834918
GCAGAGT474450.045.764829
GTTATCA43100.036.6912571
AGAGTAC464350.032.17785610-11
CAGAGTA473000.031.05589710-11
TTATCAA52600.029.5314542
AGTACGG347450.028.80123312-13
GAGTACT173300.028.7505212-13
AGTACTT175750.027.8232612-13
GAGTACG355250.027.22174512-13
GTACTTT190350.026.20474214-15
GTACGGG363950.025.67736414-15
Produced by FastQC (version 0.11.5)