FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5512372
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA205550.068.988521
GGTATCA97400.056.757991
TATCAAC291800.048.2719732
CAACGCA290750.048.1205675
TCAACGC296300.047.257684
ATCAACG300850.046.5429573
AACGCAG307450.045.6035466
ACGCAGA314300.044.4372147
CGCAGAG323400.043.2236188
GCAGAGT336950.041.2359439
AGAGTAC321700.033.33115410-11
CAGAGTA331350.029.16390610-11
AGTACGG362300.028.70663512-13
TACGGGT76050.026.24790214-15
GAGTACG371000.025.28303712-13
GTACGGG371350.024.38554814-15
TACGGGG106250.024.19112614-15
TACGGGC114350.023.4661414-15
TACGGGA134500.021.72003414-15
ACGGGAT27600.019.0780316-17
Produced by FastQC (version 0.11.5)