FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4673770
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122000.26103124458413657No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66560.1424118003239355No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA59640.1276057657950648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56010.119839016468504No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC55130.11795616814691352No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC51990.11123782299942017No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA295500.068.74121
GGTATCA190950.049.8721961
CAACGCA440200.046.7744145
TATCAAC439750.046.708182
ATCAACG449500.045.6795163
TCAACGC468450.043.769254
AACGCAG471950.043.596736
ACGCAGA485600.042.2841037
CGCAGAG484700.042.213638
GCAGAGT487850.041.7934849
ATTTAGG33450.031.125071
ACTGTGC23600.027.7607388
CTATTGA37800.026.8552369
TCTCTGC21100.024.9081848
GGCTATT41550.024.7782887
TTTAGGG42900.024.3336732
CAGAGTA474250.024.243729
GCTATTG42550.023.8572948
GGGCTAT43700.023.3934696
TAGTACT20400.023.2919644
Produced by FastQC (version 0.11.5)