FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004920319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174522
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT54790.4664876434839024No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC37370.31817198826416193No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT37010.3151069115776461No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG34810.2963758873822713No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30350.25840299287710233No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT26140.22255862384868058No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT22220.1891833443732855No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG22010.18739538297281788No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG21950.18688453685839856No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19650.1673021024723249No Hit
GGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGGAACTTA19090.16253420540441132No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC18790.15997997483231477No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG18070.15384982145928303No Hit
GGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGA17990.15316869330672395No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT17090.14550600159043423No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG16970.1444843093615956No Hit
GTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGAT15980.13605534847367695No Hit
ACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGC15740.13401196401599968No Hit
CGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTG15570.13256456669181166No Hit
GCGTAATGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACT14590.12422074682296286No Hit
GTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGGTTAGCGCAAG14120.12021911892667826No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG13700.11664319612574307No Hit
GTATAGACCCGAAACCCGGTGATCTAGCCATGGGCAGGTTGAAGGTTGGG13590.11570664491597432No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC13370.11383354249643685No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG12690.10804395319968464No Hit
GTTCCGACCTGCACGAATGGCGTAATGATGGCCAGGCTGTCTCCACCCGA12570.10702226097084602No Hit
GTGTACCCGCGGCAAGACGGAAAGACCCCGTGAACCTTTACTATAGCTTG11880.10114753065502391No Hit
GTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTT11870.10106238963595404No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)