FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4898111
Sequences flagged as poor quality0
Sequence length125
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298520.6094594426300262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103000.21028514870324497No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88040.17974276205663775No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA273300.071.758671
GGTATCA102600.057.557161
TATCAAC369250.053.2487952
TCAACGC376150.052.1223874
ATCAACG375550.052.1160623
CAACGCA376250.052.1085365
AACGCAG382050.051.3468176
ACGCAGA402500.048.696227
CGCAGAG415900.047.167718
GCAGAGT429900.044.927239
GTTATCA33450.037.2199021
AGAGTAC419850.031.31626910-11
CAGAGTA426700.030.99753610-11
GAGTACT175500.029.6700812-13
GTACTTT181300.028.3497114-15
AGTACTT177100.027.58122412-13
AGTACGG299700.027.47901512-13
GAGTACG304750.026.32437512-13
TTATCAA47350.026.1752412
GTACGGG316000.024.68618614-15
Produced by FastQC (version 0.11.5)