FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6063650
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT418940.69090399346928No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG376360.6206822623337428No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT351410.5795354283311207No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT321240.5297799180361664No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA274660.45296150008658154No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT126460.20855425362611626No Hit
GGATAACACAAATATCAGAAGTTCTTTCACAGTTAGATTGAAACTGGAAA121190.19986311874860851No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT118360.19519596282766982No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAAT116630.19234289578059421No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG113420.18704905461232096No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA111410.18373421948826202No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT98340.16217954532336135No Hit
GGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTT95370.15728150536393096No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT95180.1569681627402637No Hit
GCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATA94150.15526951588564644No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAG90480.14921705573375774No Hit
AGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCAT86220.14219158427679698No Hit
GGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCT83020.13691423482555887No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC81660.13467136130878266No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG80880.13338500738004339No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG80450.13267586354753325No Hit
AGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATG79000.13028456457744098No Hit
ATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACC78900.13011964740708978No Hit
CATAAGGATAACACAAATATCAGAAGTTCTTTCACAGTTAGATTGAAACT71400.11775085963075045No Hit
CAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATA69290.11427110733634033No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC67360.11108820594856234No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC66100.10901024960213732No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA63690.10503574579667362No Hit
AAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTA63660.10498627064556827No Hit
ATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAAT62670.10335359065909147No Hit
AAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCA62160.10251251309030039No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATCT62040.10231461248587898No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC61590.10157248521929861No Hit
GAATAGGTATCCTGAATAAATGAGAACTCTAGAAAATCAAGGTTTCAAAA61150.10084684966975335No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45800.051.0393181
ATCAACG94350.043.5626143
GTATCAA97150.043.5315931
TCAACGC95200.043.36114
CAACGCA96300.043.112875
CCTTAGC93250.039.29252
AACGCAG108200.038.7554246
ACCTTAG96750.037.4407121
ACATGGG173300.036.8280683
GTACATG186300.036.461211
TAGCCAA102750.036.064845
TACATGG182250.035.9333422
CGCAGAG117600.035.6070488
GCCCGTT4850.035.565923
GGGGTTA172200.034.95576
ATAGGGG162450.034.8940163
ACGCAGA122250.034.349987
TAGGGGT165650.034.219944
TTAGCCA110250.033.8812034
AGGGGTT167350.033.7301525
Produced by FastQC (version 0.11.5)