FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6063650
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT481480.794043191806915No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG315470.5202641973069027No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT303700.5008534463565674No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT277340.4573812802519934No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA216780.3575074418873121No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA123860.20426640719698533No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT114800.1893249115631674No Hit
GGATAACACAAATATCAGAAGTTCTTTCACAGTTAGATTGAAACTGGAAA107830.17783018478968937No Hit
GCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATA104990.17314653715171555No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT104250.17192615009111673No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAG102920.16973275172544589No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC102860.16963380142323517No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT96720.15950788716367204No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG96570.15926051140814526No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT93280.15383473650359109No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAAT92450.15246592398967618No Hit
GGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCT91340.15063534339877796No Hit
GGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTT89890.1482440444286857No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG78940.13018561427523026No Hit
AGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCAT72850.12014215860084274No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG67640.11154997402554566No Hit
AGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATG64980.10716317729420398No Hit
ATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAAT61890.10206723673035219No Hit
CATAAGGATAACACAAATATCAGAAGTTCTTTCACAGTTAGATTGAAACT61040.10066544078236705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG78750.042.233163
TCAACGC79350.041.763864
CAACGCA80150.041.496155
GGTATCA37600.041.141281
GTATCAA87000.041.0321581
ACATGGG192200.039.2826273
GTACATG208300.039.102681
TACATGG203850.038.088352
CATGGGG67850.037.6183554
ATAGGGG172700.037.1724743
AACGCAG92000.036.9209186
TAGGGGT177250.036.0504264
CGCAGAG95200.035.8551988
GGGGTTA188700.035.717596
AGGGGTT180000.035.4671865
ACGCAGA101950.033.5448537
CATGGGT39150.033.281644
GGGTTAG204200.033.2625587
CCTTAGC86750.032.9889072
ACCTTAG90500.031.490481
Produced by FastQC (version 0.11.5)