FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004920349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873529
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115931.3271454067352086No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62790.7188084196403325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52810.6045592075363267No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT49110.5622022852131984No Hit
CTTCTGGGATGTCCGCTGCAATCTGCTCCGGGGCTGCAGCAACCTCATCA35950.4115490155449905No Hit
TCCCAGAAGTGGTTGTTTCCCTTGCATGGGACGAAAGCTTGGCTCCAAAG35260.40365002192256927No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27940.3198520026238396No Hit
CTCCTGCAATGCACGCTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTT24290.2780674711429157No Hit
ACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCT24130.2762358204478615No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23720.27154221554178515No Hit
TCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAA21240.24315162976844498No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT19470.22288899395440792No Hit
TCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTG18410.21075430809967383No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18170.20800683205709258No Hit
GCCTAGCTAGAGGATCTGTGACCCCAGCCATGAGGACCCTCGCCATCCTT17990.20594622502515658No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT17730.2029697926456935No Hit
GGCCCAGGCTGAGCCACTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCC16820.19255227931757274No Hit
TTGCAGGAGAACGTCGCTATGGAACCTGCATCTACCAGGGAAGACTCTGG16370.18740076173773282No Hit
CTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCC16190.18534015470579684No Hit
CTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGCAGATTGCAGC16050.18373746034762442No Hit
ATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTG15980.1829361131685382No Hit
CTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCTTTGGAGCCAA15050.17228964350353565No Hit
GGCTGAGCCACTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGC13940.15958256680659713No Hit
GCCTGGGCCTGCAGGGCCACCAGGAGAATGGCAGCAAGGATGGCGAGGGT13780.15775091611154296No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA13270.1519125295210577No Hit
TAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTT13110.1500808788260035No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC13090.1498519224891217No Hit
CTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCTTT12880.14744788095186306No Hit
AGGTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAG12820.14676101194121774No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT12700.1453872739199271No Hit
GTATCAACGCAGAGTACGGGGCTCCTTGCTATAGAAGACCTGGGACAGAG12680.14515831758304532No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA11910.13634349861309697No Hit
CTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAG11720.13416841341272012No Hit
GAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTT11720.13416841341272012No Hit
GAAGGTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTG11660.1334815444020748No Hit
GCCATGAGGACCCTCGCCATCCTTGCTGCCATTCTCCTGGTGGCCCTGCA11590.13268019722298857No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC11530.13199332821234325No Hit
TGCCTGGAGTGGCTCAGCCTGGGCCTGCAGGGCCACCAGGAGAATGGCAG11500.1316498937070206No Hit
TTGCTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCA11380.13027615568572995No Hit
CTATTGCAGAATACCAGCGTGCATTGCAGGAGAACGTCGCTATGGAACCT10960.12546807261121268No Hit
GCCCAGAGTCTTCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGC10700.1224916402317496No Hit
GGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTGGTATT10530.12054551136825452No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC10350.11848490433631854No Hit
CTCTGGTCACCCTGCCTAGCTAGAGGATCTGTGACCCCAGCCATGAGGAC10260.11745460082035056No Hit
GAATACCAGCGTGCATTGCAGGAGAACGTCGCTATGGAACCTGCATCTAC10090.11550847195685547No Hit
GCTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACC9920.11356234309336039No Hit
GGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTT9790.11207412690362885No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG9770.11184517056674707No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG9760.11173069239830617No Hit
CTTCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGC9570.10955560719792931No Hit
TCTCTGGTCACCCTGCCTAGCTAGAGGATCTGTGACCCCAGCCATGAGGA9520.10898321635572489No Hit
GTATCAACGCAGAGTACGGGTGCTCCTTGCTATAGAAGACCTGGGACAGA9450.10818186917663866No Hit
CCCTTGCATGGGACGAAAGCTTGGCTCCAAAGCATCCAGGCTCAAGGAAA9400.10760947833443424No Hit
GAGTACGGGTGCTCCTTGCTATAGAAGACCTGGGACAGAGGACTGCTGTC9320.10669365298690713No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC9260.1060067839762618No Hit
TATTTGGGATGAGGAAAGGAAATTGAGCAGAAGGTACAGGAGTAATAGCA9160.10486200229185294No Hit
GGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCTAGAGG9150.10474752412341205No Hit
TTTGAGATGAGGAAAGGAAATTGAGCAGAAGGTACAGGAGTAATAGCAAT8910.10200004808083075No Hit
TGCTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCAC8880.10165661357550808No Hit
CTTTGGAGCCAAGCTTTCGTCCCATGCAAGGGAAACAACCACTTCTGGGA8850.10131317907018543No Hit
GGCTCAGCCTGGGCCTGCAGGGCCACCAGGAGAATGGCAGCAAGGATGGC8780.10051183189109922No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)