FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4139910
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145710.3519641731341986No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88360.21343459157324673No Hit
TTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80640.19478684319224332No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52760.12744238401317903No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCAA78900.062.7136231
GTATCAA142800.061.7974931
ATCAACG267450.051.559613
TTACGGG25200.051.4258841
TCAACGC270850.051.056754
TATCAAC273550.050.5534172
AACGCAG275150.050.2996186
CAACGCA276100.050.1671875
GGTATCA51800.050.14431
ACGCAGA289350.047.8117527
CGCAGAG296200.046.7439238
TAGTACG23100.044.6864851
GCAGAGT312900.044.284989
TGTATCA34150.034.4983941
AGAGTAC307450.033.89373410-11
AGTACGG221800.029.34009412-13
CAGAGTA313550.028.86870210-11
AGTACTT148450.026.0755812-13
GAGTACG214200.025.91560712-13
GAGTACT136750.025.43486412-13
Produced by FastQC (version 0.11.5)