FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4751710
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA169250.069.853081
GGTATCA71100.063.0983621
ATCAACG233600.049.742483
TCAACGC237500.048.975764
CAACGCA238600.048.7682155
TATCAAC244400.048.0566062
AACGCAG244900.047.5922556
ACGCAGA251050.046.309417
CGCAGAG253400.045.8564728
GCAGAGT275550.042.0641869
AGAGTAC264500.034.14299410-11
AGTACGG300000.029.77501712-13
CAGAGTA272450.029.41402410-11
GAGTACG302800.026.2197612-13
TACGGGG94450.025.85107814-15
TACGGGT55650.024.79649214-15
GTACGGG317950.024.44094714-15
TACGGGC75100.024.43333214-15
TACGGGA129150.024.13261214-15
ACGGGAC30700.019.4751116-17
Produced by FastQC (version 0.11.5)