FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6062323
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC184020.3035470066507509No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158120.2608241098338046No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT131830.21745789526556075No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT122240.20163887671442118No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT109280.1802609329789917No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT95400.15736541916357805No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG90000.1484579426071491No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC75000.12371495217262427No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC69150.11406518590315956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67640.11157439153275074No Hit
GGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGA64090.10571855046324652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61600.1016112140511154No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG61330.10116584022329393No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA409550.074.762061
GGTATCA182750.061.776641
TATCAAC575900.053.079522
ATCAACG615450.049.571993
TCAACGC621450.049.0451744
AACGCAG633450.048.0961156
CAACGCA642000.047.4289975
ACGCAGA652150.046.661567
CGCAGAG657450.046.2940568
GCAGAGT679850.044.7949569
AGAGTAC674650.033.1726910-11
CAGAGTA679850.030.66435810-11
GAGTACT98950.026.8683412-13
AGTACGG725750.026.81763312-13
ACGGGTA36650.025.29413616-17
TACGGGT180550.024.8387814-15
GAGTACG732550.024.81691212-13
TACGGGC175700.024.39038314-15
GGGCTAT32350.023.5076946
GTACGGG762250.022.76121314-15
Produced by FastQC (version 0.11.5)