FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6062323
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC228020.37612644525869043No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT123630.20393172716135383No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT109710.18097023203811477No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT96520.15921289578268924No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT89870.14824350335671657No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG82770.13653182121770813No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC80960.1335461670386088No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA79340.1308739240716801No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC76130.1255789241186918No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG70500.11629205504226679No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG68130.11238266255361186No Hit
GGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGA62280.10273289628414718No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA341900.073.702631
GGTATCA158950.059.8197941
TATCAAC503200.049.993352
ATCAACG541900.046.3242343
TCAACGC548350.045.7351884
AACGCAG551650.045.478426
ACGCAGA562850.044.481317
CGCAGAG565300.044.3186238
CAACGCA570400.043.9513175
GCAGAGT581400.043.043079
AGAGTAC577550.033.6877810-11
CAGAGTA583100.029.57298310-11
AGTACGG726250.026.77337312-13
TACGGGC168700.026.00265314-15
TTTAGGG34250.025.363562
GGGCTAT33900.025.2593566
TTAGGGC38900.024.6260193
GCTATTG34300.024.098758
ACTGTGC140250.023.998688
GAGTACG730000.023.57672912-13
Produced by FastQC (version 0.11.5)