FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6493589
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT356270.5486488288679804No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA216850.33394475689791886No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT166310.2561141458136633No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT156310.24071434148357712No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG151860.23386142855668876No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146360.22539153617514135No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG97360.14993249495771907No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT95680.14734532783026458No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95200.14660613722242044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83930.1292505577424133No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA69790.10747523441967145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66920.10305549057693672No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94700.063.889381
CAACGCA102900.056.325225
TCAACGC104600.055.409374
AACGCAG105550.054.638236
ATCAACG108500.053.550413
CGCAGAG115800.049.7386258
ACGCAGA116900.049.3949137
CCGTGCG1057.304152E-847.997779
GGGTTAG121700.047.9200027
GTACATG61200.047.8971021
GGTATCA48700.047.885961
ATAGGGG116350.046.534013
CCGCAGT8950.045.175151
CGCAGTA9500.043.9572072
GGGGTTA133600.043.7055666
CATGCTA64300.042.5492254
TAGGGGT128450.041.9262964
GGACCGT4200.041.1418956
AGGGGTT129650.041.0387655
GCAGAGT140450.040.7528279
Produced by FastQC (version 0.11.5)