FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4203429
Sequences flagged as poor quality0
Sequence length125
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173590.41297236137448734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65250.15523040831663865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58760.1397906328380948No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC43260.10291597645636455No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA162200.071.652681
ATCAACG255500.054.7330933
TATCAAC256300.054.7168162
TCAACGC257000.054.653174
AACGCAG262550.053.5406156
CAACGCA264100.053.183895
GGTATCA61400.052.9192161
ACGCAGA277150.050.821397
CGCAGAG284150.049.6089068
GCAGAGT298300.047.1428159
CTATCAA52850.043.1106151
CGTATCA8650.038.2771031
AGAGTAC290150.033.98105610-11
CTACGGG13450.033.7960471
CAGAGTA294250.030.37994610-11
AGTACGG222150.030.07258412-13
GAGTACT112800.027.03822312-13
GAGTACG222000.026.51613212-13
TACGGGC55900.025.24723814-15
AGTACTT129200.024.51822912-13
Produced by FastQC (version 0.11.5)