FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5892819
Sequences flagged as poor quality0
Sequence length150
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA231130.39222314481405246No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC194030.32926516154662144No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA149530.25374952123932537No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC141450.24003791733633767No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC136770.23209604774896364No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG135460.2298730030567713No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC125570.2130898641210599No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC119690.20311161771641045No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA118300.20075281456973312No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC115150.1954073254243852No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT98680.16745805360728033No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA96210.16326651132505512No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC95740.16246893040495558No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC82930.1407306078805407No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA82520.14003484580130493No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA82290.1396445402446605No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG81200.137794831302302No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC79840.13548693757605657No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA77560.1316178216232333No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA59310.1006479241938366No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA159050.082.286331
GGTATCA72300.070.602241
ATCAACG226000.057.8289263
AACGCAG229750.057.2294166
CAACGCA233900.056.337025
ACGCAGA234450.056.3275767
CGCAGAG235150.056.0093358
TCAACGC233700.055.831234
GCAGAGT256100.051.1747679
TATCAAC276350.047.9421772
ACTGTGC63350.032.814698
CAGAGTA256100.031.1767239
TTAGGGC93850.030.8099083
AGGGCTA88000.030.3242385
TAGGGCT98750.030.0823384
GCTATTG88500.029.5852478
CTATTGA90900.029.3580449
TTTAGGG93900.028.5713542
GGGCTAT100600.027.0981646
TATTCTC68550.026.4418415
Produced by FastQC (version 0.11.5)