FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004920515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2935735
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141980.48362675786472553No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT110360.3759194886459438No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83310.28377901956409557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71340.2430055846321279No Hit
CTTCTGGGATGTCCGCTGCAATCTGCTCCGGGGCTGCAGCAACCTCATCA62680.21350700931793912No Hit
TCCCAGAAGTGGTTGTTTCCCTTGCATGGGACGAAAGCTTGGCTCCAAAG56930.1939207728217976No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53480.1821690309241127No Hit
CTCCTGCAATGCACGCTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTT46650.15890398826869592No Hit
ACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCT45370.15454392170955483No Hit
GGCCCAGGCTGAGCCACTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCC35560.12112809909613778No Hit
GCCTAGCTAGAGGATCTGTGACCCCAGCCATGAGGACCCTCGCCATCCTT33550.11428143207748656No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32480.11063668893820458No Hit
TCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTG31310.10665131559898969No Hit
TCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAA30880.10518660573927824No Hit
CTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCC30800.10491410157933193No Hit
CTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGCAGATTGCAGC30120.10259781621978824No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)