FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11577413
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1612731.392996863807139No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT1083110.935537153248312No Hit
ACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA1025160.8854827930903043No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG638030.5510989372150756No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT580860.5017183026985389No Hit
CCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAGTATAG521430.4503855913233812No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT446400.38557836711880283No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA312120.26959390668709843No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT308880.26679535402252647No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA285980.24701546018959503No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT279820.24169475512361874No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC277330.23954401557584581No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA276030.23842113950672747No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG264420.22839299245867797No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT261940.22625089041912902No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT235070.20304190582127457No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT227570.19656377465328395No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT226730.19583822396246897No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG225840.19506948573053412No Hit
GGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTT221520.1913380821777715No Hit
GCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATA219890.18993016833726153No Hit
ATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAAT217940.18824585423358398No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG217090.18751166603454503No Hit
CTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGAT215560.18619012727627493No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA214640.1853954765196681No Hit
AGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCAT213510.1844194380903575No Hit
GGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCT209630.18106808489945034No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT204980.17705164357529615No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT198690.171618650902408No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAG198210.17120405050765658No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG189810.16394854359950709No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT188990.1632402679251401No Hit
GTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGT187480.16193600418331797No Hit
TCTGAGATGTTAGTGTTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC181220.1565289240351018No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT179600.15512964770281581No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC178760.15440409701200086No Hit
GTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTTTATGTTGT174750.15094045621418187No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC172800.1492561421105043No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAAT172310.14883290420752893No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC169510.14641440190481242No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC168130.14522242576990213No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC165190.1426829983520498No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGA164160.1417933350049791No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG160440.13858018194565574No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGA156920.13553977905081213No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTA155110.13397639006227038No Hit
GGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCG154370.1333372144536953No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACTCTA154130.1331299142563196No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA151050.13046956172333146No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAA147760.12762782151763957No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT144250.12459605613101994No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC142920.1234472675372296No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT139370.12038095211771405No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA139080.12013046437921839No Hit
TTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATT138980.12004408929697852No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA136010.11747874935445424No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGC129350.11172616887727854No Hit
AAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCA128540.11102653071113555No Hit
GTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATG127180.10985182959267326No Hit
GGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTGTTA125760.10862530342486702No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT125670.10854756585085114No Hit
ATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTA125190.10813296545609974No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC125030.10799476532451593No Hit
TCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTT123080.10631045122083836No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTT120710.10426336177175333No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT117960.10188804701015677No Hit
ACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGG117550.10153390917297327No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC117140.10117977133578977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGC169500.099.3524862
GTACATG175850.096.35491
CATGCTA190100.088.623364
ACATGCT193500.087.139443
GGTATCA47700.082.591761
TAAGACT211450.080.0155949
TGCTAAG251250.067.0828866
CCTGGGG24850.066.9259343
CCCTGGG25200.065.9978262
GGGGTTA460800.059.1696746
CAACGCA183050.058.3294335
GGGTTAG475550.057.1981627
ATCAACG187050.057.1580733
TTGTACG27100.057.1194049
TCAACGC188300.056.741144
AACGCAG188450.056.6962176
CTCTAAT54650.056.2156641
ACCCTGG30850.056.001711
CGGGACA23550.055.946724
CGCAGAG192950.055.2615248
Produced by FastQC (version 0.11.5)