FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004920530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2743103
Sequences flagged as poor quality0
Sequence length150
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT192940.7033640370048081No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG98390.35868139111072384No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA95670.3487656132489374No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT74870.27293907665880573No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT68780.2507379416667912No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT60020.21880330414133192No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58880.214647426655142No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA57690.21030927384061043No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC42100.15347582646368002No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG41540.1514343427862534No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA40320.1469868247747168No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT40040.1459660829360035No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT38340.13976872177238697No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG37220.13568575441753372No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC35020.12766563997050057No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC33830.12332748715596897No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT33500.12212446998891402No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC32700.11920806473544741No Hit
GAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTG32640.11898933434143742No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAG32380.11804150263406077No Hit
GTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATAT31700.11556255816861415No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT30920.11271906304648421No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT30450.1110056749600726No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACATTCTCCTGA29690.1082350899692793No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT29440.107323713327571No Hit
TCTTTATCACCAGAATGAGGAACTCCTGGAAGTTAACTGCACCATCAGTG28810.10502704419046605No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG27820.10141799268930114No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)