FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004920550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4033516
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA558321.3842017733411742No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC340520.8442262284319685No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC305870.758321028105504No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC296280.7345452453888865No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC279910.6939603065910734No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA278230.6897952059691842No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA264790.6564744009940707No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC254510.6309879519506059No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG240820.5970473403353302No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC226470.5614704391900267No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA217190.5384632167072103No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG202210.5013244028286983No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT181760.45062421966344No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC177480.4400131299838652No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC176080.43654221279895755No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG166350.4124193383638493No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA159670.3958581049387184No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT149790.37136334651951297No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC146960.3643471353528782No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC141780.3515047417687199No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT140860.34922385333292344No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA137660.34129032833884876No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA127720.3166468163260044No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA127170.3152832417176478No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG123550.3063084415681009No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA120050.29763114860583173No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT120040.29760635634022525No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC119960.2974080182153734No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT118630.2941106468897111No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT115510.2863754600204883No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG114800.284615209162428No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA111930.27749982893336733No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC111770.2771031526836636No Hit
ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG111460.2763345924498626No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA110110.27298763659298736No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA103710.25712058660483805No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG102660.25451739871615736No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT96430.23907181724331822No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA95700.23726198185404496No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA90460.22427083467624773No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA88080.21837027546190468No Hit
ATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACT83980.20820544656324655No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA83170.2061972730491214No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT79570.1972720574307874No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC79490.19707371930593556No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG78250.19399947837073164No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG74760.18534697767406896No Hit
GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT74030.18353714228479567No Hit
ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA73960.1833635964255503No Hit
GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG73240.18157855330188352No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA72670.18016539416231397No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC69660.17270292221476252No Hit
ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA67080.1663065176882898No Hit
TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA66880.16581067237616015No Hit
CTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAG65860.16328186128429886No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT65100.16139764909820611No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA64190.15914155292801616No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC63980.15862091535028No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT62350.15457977605642323No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT61350.1521005494957749No Hit
GTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATC61310.152001380433349No Hit
CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT59960.14865442457647374No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG58790.14575372950051518No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT58390.14476203887625586No Hit
ATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACT58340.14463807754822344No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA58110.14406785543927433No Hit
ATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTT55820.13839042661538964No Hit
CTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTA54650.1354897315394311No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA54490.13509305528972737No Hit
GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT53420.13244028286983367No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA52840.13100233146465765No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC52370.12983709498115292No Hit
ATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAA52280.12961396459069457No Hit
ATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCT49130.12180440092465233No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG48960.12138293240934211No Hit
TATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTG48010.11902766717672622No Hit
TATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTT46960.11642447928804547No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA46790.11600301077273525No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA46570.11545758092939262No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC45480.11275522397828594No Hit
GTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCA45290.11228417093176275No Hit
GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA45140.11191228694766552No Hit
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG44910.1113420648387164No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC44590.11054871233930892No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG44410.11010245155839223No Hit
TGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTT44220.10963139851186904No Hit
TGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTA44020.10913555319973939No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT43840.10868929241882269No Hit
AACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCC43710.1083669929659384No Hit
AACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTT43480.10779677085698929No Hit
CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT43450.10772239406016983No Hit
AATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATT42930.10643319624863272No Hit
GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT42750.105986935467716No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC42260.10477211445299832No Hit
AATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGT42140.10447460726572054No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG41670.10330937078221582No Hit
ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC41550.10301186359493802No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT41480.10283831773569263No Hit
CTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGG41380.10259039507962779No Hit
TTTCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCCAACTAT40700.10090452101838693No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)