FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5761355
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT113700.19734940825552322No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG87020.15104085757603897No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA82780.14368147770793505No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC78850.13686016570754622No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA74010.1284593641599936No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT71700.12444989069411623No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT69280.12024948992033992No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT68280.11851378712125879No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC66920.11615323131450848No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG65050.11290746708022678No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA64060.11118912130913647No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA63940.11098083697324675No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA63850.11082462372132944No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT62910.1091930630901932No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC62810.10901949281028507No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC61090.10603408399586554No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT59920.10400331172094066No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC58520.10157332780222708No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCG58400.10136504346633735No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA195350.051.2485961
GGTATCA107950.046.1777341
ATCAACG242550.040.5889633
CAACGCA248100.039.7001345
AACGCAG252150.039.3110166
TCAACGC253400.038.967064
ACGCAGA255350.038.7019467
CGCAGAG255500.038.7008178
GCAGAGT264500.037.517549
TATCAAC270250.036.7588582
GTACTGG34650.032.7899971
AGAGTAC268550.028.20014210-11
CTGGTTC39950.027.3966334
CAGAGTA269500.027.27424410-11
GGTTCAC46200.025.7410146
TGGTTCA43050.025.6979315
GTTCACT46800.025.1568937
CAGTACG2001.8394749E-623.7828399
TACGGGT41600.023.1642284
AGTACGG324550.023.06893312-13
Produced by FastQC (version 0.11.5)