FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4590134
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165730.36105699746456205No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94710.2063338455914359No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA86660.18879623122113645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71530.15583423054751777No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC62770.1367498203756143No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC62010.13509409529220714No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA58020.12640153860431963No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC47270.10298174301665267No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA205000.067.2557141
GGTATCA111200.047.0832251
CAACGCA328000.042.6466675
TATCAAC335750.041.5224952
AACGCAG342050.041.118976
ATCAACG345950.040.5387233
CGCAGAG353650.039.568618
ACGCAGA354100.039.518337
TCAACGC358350.039.2018664
GCAGAGT374350.037.4282539
AGAGTAC361400.033.43409710-11
ATTTAGG41150.028.3061831
CAGAGTA368700.027.8325359
AGTACTT117350.026.48208412-13
AGTACGG341200.026.21834812-13
TTTAGGG44400.025.965112
GGGCTAT45450.025.3630476
CTATTGA49250.023.6474029
GCTATTG49400.023.5755968
TACGGGG102150.023.46377414-15
Produced by FastQC (version 0.11.5)