FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5228087
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108860.20822147756913764No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC67810.1297032738743636No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC65110.1245388609638669No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53650.10261879727709199No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA52940.1012607479561836No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA148100.079.312071
GGTATCA62000.075.828371
TCAACGC187100.061.9190984
CAACGCA189950.061.069965
ATCAACG190250.060.9666633
AACGCAG192650.060.1766856
ACGCAGA201150.057.6695987
CGCAGAG201650.057.4908948
TATCAAC208400.056.107682
GCAGAGT233750.049.6883059
CAGAGTA235150.030.6827419
AGTACGG198850.025.1647852
GAGTACG198850.025.1432861
TACGGGT53400.025.079224
ACTGTGC50800.024.8053728
TTAGGGC45250.022.4347953
GTACGGG214750.022.3621853
CTGTGCA56900.021.8929989
AGAGTAC217100.021.76087610-14
ACGGGTT18000.021.6019365
Produced by FastQC (version 0.11.5)