FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5228087
Sequences flagged as poor quality0
Sequence length150
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC67940.1299519307922764No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA56990.10900736732192866No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC56620.10829965147863836No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52870.10112685576961515No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA115750.074.699151
GGTATCA56650.060.897381
TCAACGC144100.059.5100064
CAACGCA147900.058.4213945
ATCAACG148650.057.833793
AACGCAG149350.057.757776
ACGCAGA154800.055.721117
CGCAGAG154600.055.4206248
TATCAAC168150.050.9972
GCAGAGT180800.047.3089839
CAGAGTA181900.031.5465039
GAGTACG162750.026.4740811
AGTACGG163850.026.2335852
GTATAGG49500.026.1718851
ACTGTGC48650.025.1593828
TACGGGT43450.024.0281564
GTAGGAC23000.023.4787863
GTACGGG177400.023.4593753
TATACCG5950.022.992995
TACGGGC30950.022.7985734
Produced by FastQC (version 0.11.5)