FastQCFastQC Report
Fri 9 Sep 2022
EGAF00004920655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1615630
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC91380.5655997969832202No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT54960.34017689693803654No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG50530.3127572525887734No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT47300.29276505140409625No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT36610.2265989118795764No Hit
GGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGA35030.21681944504620487No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT34690.21471500281623887No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT32700.20239782623496716No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG30100.1863050327116976No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAAAAAAAAAAAAAAAAAAAAAAA29800.1844481719205511No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC28150.17423543756924542No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28070.17374027469160638No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG27380.16946949487196944No Hit
GTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGAT25050.15504787606073173No Hit
GGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGGAACTTA24820.1536242827875194No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG24490.15158173591725826No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG22480.13914076861657682No Hit
TGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGAGAGCAAGCGGAC22220.13753148926424985No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC22000.13616979135074242No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA21870.13536515167457897Clontech Universal Primer Mix Long (96% over 26bp)
GTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACT21870.13536515167457897No Hit
GTATAGACCCGAAACCCGGTGATCTAGCCATGGGCAGGTTGAAGGTTGGG21850.1352413609551692No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG21500.13307502336549829No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACG21000.12998025538025415Clontech Universal Primer Mix Long (96% over 25bp)
GTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTT20940.12960888322202485No Hit
CGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTG19980.12366692869035609No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT19700.12193385861861937No Hit
AACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAA19200.11883909063337521No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG19010.11766307879898244No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT19000.11760118343927756No Hit
ACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGC18370.11370177577786993No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT17800.11017374027469161No Hit
CTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCC17600.10893583308059394No Hit
CTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTC17120.10596485581475956No Hit
GTTCCGACCTGCACGAATGGCGTAATGATGGCCAGGCTGTCTCCACCCGA16770.10379851822508866No Hit
GTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGGTTAGCGCAAG16570.10256061103099101No Hit
GCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCGGGGTT16270.10070375023984453No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)