FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3732934
Sequences flagged as poor quality0
Sequence length150
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA163820.4388505127602042No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC157710.4224826905592223No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC131890.3533145777557278No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA111610.2989873381099157No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104320.2794584635035069No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT99140.26558197921527676No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC90600.242704532145492No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC90040.24120437168189957No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC85440.2288816250166759No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC80920.21677318698910833No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT72020.19293135104987122No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA71580.19175265354276289No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG68700.1840375425871446No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA67380.18050145006581955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61300.16421399360395872No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC56290.15079291517074772No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC52170.13975602033146045No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC52030.13938098021556233No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC51970.1392202487373203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48070.1287727026515872No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA47980.12853160543422412No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA44980.12049503152212174No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC44360.11883413958028725No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC43730.11714645905874575No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT43280.1159409729719304No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA41100.11010106259580267No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG40180.10763651326275792No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT38860.10410042074143289No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA38310.10262704885754745No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58700.087.955951
GTATCAA126500.083.11911
CGCAGAG180900.057.6595658
ACGCAGA182600.057.5545587
CAACGCA184400.057.187795
ATCAACG183350.056.6914023
AACGCAG188400.056.2408836
TATCAAC188650.055.7424352
GCAGAGT186950.055.524269
TCAACGC202900.051.6899074
TATTCTC39850.046.030125
TTCTCTG41150.043.876727
TCTCTGC41450.043.733878
ATTTAGG75900.042.706021
CTATTGA78750.042.202039
ATTCTCT44400.041.1510626
AGGGCTA80550.040.632745
ACTGTGC64400.040.3239638
GCTATTG83600.039.323478
CTGTGCA49400.039.171239
Produced by FastQC (version 0.11.5)