FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6274226
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTT137960.2198836956144073No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101240.16135854844884454No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT96070.15311848824062124No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG92310.1471257171800952No Hit
CTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACC88440.14095762568960699No Hit
CTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCA77600.12368059422787767No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA74380.11854848709625697No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT73020.11638088905308798No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC71350.11371920616184371No Hit
AAATAAATGTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATA69380.1105793766434298No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66250.10559071349995998No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC65170.10386938564214933No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA188550.041.0775761
GGTATCA111550.037.1159061
ATACGAC5800.036.921943
TCAACGC238800.033.2051774
ATCAACG239350.033.128883
CAACGCA243500.032.5886845
AACGCAG249200.031.7952866
TATCAAC257400.030.8526952
ACGCAGA291000.027.2690127
CGCAGAG290700.027.2562278
CGACACT7550.026.0000116
GCAGAGT313050.025.3864969
GTCACGT2601.1114025E-925.1668789
TACGACA8100.024.234774
TCTATAC37550.023.4456233
AGAGTAC312800.023.1817910-11
CATGGGT51450.022.7767034
GGGGTTA31400.022.7330746
GGGTTAG33750.021.8551837
TACCAAC27350.021.3145077
Produced by FastQC (version 0.11.5)