FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6274226
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC128080.20413673335962076No Hit
TCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTT127680.20349920452339462No Hit
CTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACC86940.1385668925537588No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG83430.13297257701587414No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA81830.13042246167096944No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT77010.12274023919444407No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT73650.1173849969701442No Hit
CTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCA71390.11378295904546631No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT68960.10990997136539231No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA170300.038.7099761
CAACGCA186400.034.376975
TCAACGC185600.034.364864
ATCAACG186650.034.2352943
AACGCAG186500.034.160586
ATACGAC6850.031.2686943
TATCAAC208950.030.8663042
ACGCAGA213250.029.8177957
CGCAGAG213000.029.7380088
GGTATCA103100.028.681081
GGGGTTA37150.028.181786
GCAGAGT233450.027.3877549
GTACATG321150.026.6964681
CATGGGT54850.026.1419624
TAGGGGT40550.026.1172184
TACATGG324200.025.8396972
ACATGGG311150.025.7761383
AGGGGTT39700.025.627335
GGGTTAG41350.025.6053897
TACGACA9150.025.3595354
Produced by FastQC (version 0.11.5)