FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5468592
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT89040.16282070412274313No Hit
GTATTTCACTGTCTCTTATACACATCTGACGCTGCCGACGATCTACTCTG75840.13868286388891327No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA132900.064.458691
GGTATCA57650.054.3820081
TCAACGC169800.049.715134
AACGCAG170100.049.4176146
CAACGCA170650.049.258345
ATCAACG172050.048.9612463
ACGCAGA176500.047.659417
CGCAGAG177100.047.531538
TATCAAC195800.043.660432
GCAGAGT200450.042.0243579
AGAGTAC195500.034.67460310-11
TAGGGGT41100.034.5939374
ATAGGGG44300.031.4236093
AGTACGG223100.030.09164412-13
AGGGGTT46400.029.7449975
GGGTTAG47550.028.9005017
CAGAGTA203950.028.29384210-11
GTATAGG76100.026.5007971
GAGTACG221450.025.78929512-13
TACGGGT66250.025.68171714-15
Produced by FastQC (version 0.11.5)