FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5526402
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA93180.1686087982741755No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC81400.14729294032536902No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC80850.14629771775560302No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC80760.14613486315327767No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC78430.14191873844863256No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT77230.1397473437509613No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT76620.1386435514463117No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCG74550.13489789559282875No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCA72130.13051891628585832No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT71520.12941512398120875No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG70320.12724372928353747No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA65190.11796101695099272No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA60510.10949257763007468No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA58940.10665166956728808No Hit
GTATCAACGCAGAGTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTT58460.10578311168821958No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAG57450.1039555211510129No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTT57300.10368409681380399No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG56760.10270696919985191No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91550.076.588871
GTATCAA190050.063.3587651
ATCAACG258000.046.154743
TATCAAC267100.045.005372
CAACGCA267500.044.4881255
TCAACGC270800.044.039024
AACGCAG272600.043.9532936
ACGCAGA277850.042.885837
CGCAGAG279700.042.6021738
GCAGAGT297900.039.938829
AGAGTAC288250.031.91432610-11
CAGAGTA302800.030.74398810-11
GTACTGG40750.026.8762631
GAGTACG367000.025.12291712-13
AGTACGG367650.024.83596212-13
TACTGGT42700.024.5164032
GACCGTT14300.024.1167347
ACGGGTT17550.023.0437225
TATATCG3103.4015102E-1023.0218585
ACTGGTT45900.022.5480253
Produced by FastQC (version 0.11.5)