FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004920711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58064136
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT3418570.5887575766218238No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT3139030.5406142614435872No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT3044740.524375321799329No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA2275320.391863232064626No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT2117450.36467433184573694No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC1990150.34275029942751584No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC1824380.3142008347459092No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT1727250.29747278078847156No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG1724180.29694405510485855No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA1703280.29334458709589684No Hit
CATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGT1698000.2924352478094223No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT1666390.2869912677250549No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT1636120.2817780669293004No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1592690.27429840685134793No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC1444020.24869396144980094No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG1398240.2408095765000275No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC1389040.23922512168268548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1356630.23364336291854926No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC1293980.22285357005914977No Hit
ACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGG1172180.20187676606433963No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1123870.1935566560397971No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC1107790.19078730457644286No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG1044900.1799561781131127No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA1044530.17989245547371963No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG1029250.17726088269013424No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA1006300.17330835681426482No Hit
TCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTA974360.16780754302449277No Hit
ATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAA973200.16760776393882793No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA960180.1653654159255896No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC918280.15814925757269513No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG880760.15168743749153524No Hit
ACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGG876360.15092965475280645No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA872730.1503044839933552No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG872240.15022009455199678No Hit
GTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAAC863730.14875447384595544No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT860840.1482567483652904No Hit
TACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTG860800.14824985943130198No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC819790.14118697985965037No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAG791110.13624761418993644No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT781670.1346218257686638No Hit
GATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCC747460.1287300649750476No Hit
TTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACT732600.12617082599834087No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG705490.12150185098767335No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA698610.1203169543416611No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT673800.11604409303532907No Hit
GTTTTCATGTTACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATT671720.11568586846793003No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT669940.11537931090544427No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT631310.10872632290610507No Hit
ACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA631280.10872115620561373No Hit
ATACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGT628330.10821309732396604No Hit
GTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTT625520.10772914971127788No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG617600.10636514078156609No Hit
CTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC607340.10459812921353037No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTGCCGAGTT606700.10448790626971527No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA605270.1042416268796284No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT598670.10310495277153525No Hit
GGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTT592690.10207505714026296No Hit
TTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTT586310.10097627216910623No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)