FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004920732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666523
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA81680.30631650280158845Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT71390.2677269237880191Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT38400.14400775841798477No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG33340.12503173608478158Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG31730.11899391079694419Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGC31330.11749382998009017No Hit
GTATCAACGCAGAGTACATGGGAAAGAGTGTCGTATATCTGTCTCTTATA28560.10710577032337616No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT27450.1029430460566063No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)