FastQCFastQC Report
Sat 10 Sep 2022
EGAF00004920773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1617314
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCTGAACCGCTCTTCCGATCTAAGCCTTCCCCTATGTTCAAATGTTGC205161.2685229955345716Illumina Paired End PCR Primer 2 (100% over 23bp)
CCCTGGGAAATGTCACCTCTTGGCCCCAGAAGATTCCCCAAAGCACAAAA116280.7189698475373366No Hit
ACGCTCTTCCGATCTCCCTGGGAAATGTCACCTCTTGGCCCCAGAAGATT100900.6238739045108124No Hit
TCCCTACACGACGCTCTTCCGATCTCCCTGGGAAATGTCACCTCTTGGCC98500.6090344855729932Illumina Single End PCR Primer 1 (100% over 25bp)
CTTCCGATCTCCCTGGGAAATGTCACCTCTTGGCCCCAGAAGATTCCCCA96330.5956171776167151No Hit
ATCTAAGCCTTCCCCTATGTTCAAATGTTGCTTACAGACCTGTGTCCTGT91130.5634651032514404No Hit
GCTGTATCTTGTGGAAAACCAGGACCATCATCTCAGCTTTTGTGCTTTGG81350.5029944710798274No Hit
GTTCAGGACCCAGGTATCAGCTGTATCTTGTGGAAAACCAGGACCATCAT74770.4623097308253067No Hit
CTACACGACGCTCTTCCGATCTCCCTGGGAAATGTCACCTCTTGGCCCCA73170.45241678486676057Illumina Single End PCR Primer 1 (100% over 22bp)
GTTCAAATGTTGCTTACAGACCTGTGTCCTGTTCAGGACCCAGGTATCAG72720.4496343938159195No Hit
GGTATCAGCTGTATCTTGTGGAAAACCAGGACCATCATCTCAGCTTTTGT61970.38316616315693797No Hit
ACACGACGCTCTTCCGATCTCCCTGGGAAATGTCACCTCTTGGCCCCAGA60930.376735748283883No Hit
CCTGTGTCCTGTTCAGGACCCAGGTATCAGCTGTATCTTGTGGAAAACCA58850.3638749185377731No Hit
TTACAGACCTGTGTCCTGTTCAGGACCCAGGTATCAGCTGTATCTTGTGG46960.2903579638833275No Hit
CCCCAGAAGATTCCCCAAAGCACAAAAGCTGAGATGATGGTCCTGGTTTT45750.28287642350217707No Hit
AGATACAGCTGATACCTGGGTCCTGAACAGGACACAGGTCTGTAAGCAAC41140.2543723729591162No Hit
CTTCCGATCTAAGCCTTCCCCTATGTTCAAATGTTGCTTACAGACCTGTG39860.2464580161922793No Hit
TTCCCTACACGACGCTCTTCCGATCTCCCTGGGAAATGTCACCTCTTGGC38150.2358849301990832Illumina Single End PCR Primer 1 (100% over 26bp)
GCTGATACCTGGGTCCTGAACAGGACACAGGTCTGTAAGCAACATTTGAA36580.22617747697725984No Hit
GATCTAAGCCTTCCCCTATGTTCAAATGTTGCTTACAGACCTGTGTCCTG36030.22277677680400962No Hit
TCCGATCTCCCTGGGAAATGTCACCTCTTGGCCCCAGAAGATTCCCCAAA36000.22259128406728687No Hit
CCCCTATGTTCAAATGTTGCTTACAGACCTGTGTCCTGTTCAGGACCCAG34870.21560439098406373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34630.21412044909028177No Hit
CCCCAAAGCACAAAAGCTGAGATGATGGTCCTGGTTTTCCACAAGATACA33780.2088648215498042No Hit
GGGAAATGTCACCTCTTGGCCCCAGAAGATTCCCCAAAGCACAAAAGCTG31130.19247962980596223No Hit
GCACAAAAGCTGAGATGATGGTCCTGGTTTTCCACAAGATACAGCTGATA30940.1913048424733849No Hit
GTCCTGAACAGGACACAGGTCTGTAAGCAACATTTGAACATAGGGGAAGG30840.19068653335097577No Hit
CCGCTCTTCCGATCTAAGCCTTCCCCTATGTTCAAATGTTGCTTACAGAC30700.18982090057960296No Hit
CCTCTTGGCCCCAGAAGATTCCCCAAAGCACAAAAGCTGAGATGATGGTC30520.18870794415926653No Hit
CCCTACACGACGCTCTTCCGATCTCCCTGGGAAATGTCACCTCTTGGCCC29110.1799897855332978Illumina Single End PCR Primer 1 (100% over 24bp)
CTCTTGGCCCCAGAAGATTCCCCAAAGCACAAAAGCTGAGATGATGGTCC28110.1738066943092065No Hit
GCCTTCCCCTATGTTCAAATGTTGCTTACAGACCTGTGTCCTGTTCAGGA24760.15309333870850064No Hit
CTCTTCCGATCTCCCTGGGAAATGTCACCTCTTGGCCCCAGAAGATTCCC23570.14573546015183197No Hit
GACCTGTGTCCTGTTCAGGACCCAGGTATCAGCTGTATCTTGTGGAAAAC23000.14221109815409994No Hit
ATACAGCTGATACCTGGGTCCTGAACAGGACACAGGTCTGTAAGCAACAT22690.14029433987463164No Hit
CAGCTGTATCTTGTGGAAAACCAGGACCATCATCTCAGCTTTTGTGCTTT22390.13843941250740424No Hit
ATGTTCAAATGTTGCTTACAGACCTGTGTCCTGTTCAGGACCCAGGTATC21320.13182350489762656No Hit
CTTCTGGGGCCAAGAGGTGACATTTCCCAGGGAGATCGGAAGAGCGTCGT20750.12829914289989452No Hit
ATCTTGTGGAAAACCAGGACCATCATCTCAGCTTTTGTGCTTTGGGGAAT20330.12570224458577617No Hit
AACCAGGACCATCATCTCAGCTTTTGTGCTTTGGGGAATCTTCTGGGGCC20320.12564041367353526No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT19520.12069394069426222No Hit
CCACAAGATACAGCTGATACCTGGGTCCTGAACAGGACACAGGTCTGTAA19080.11797338055566205No Hit
CCTATGTTCAAATGTTGCTTACAGACCTGTGTCCTGTTCAGGACCCAGGT18350.1134597239620754No Hit
GTCCTGGTTTTCCACAAGATACAGCTGATACCTGGGTCCTGAACAGGACA17790.10999719287658426No Hit
GTTGCTTACAGACCTGTGTCCTGTTCAGGACCCAGGTATCAGCTGTATCT17710.10950254557865696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17470.10801860368487505No Hit
GTCCTGTTCAGGACCCAGGTATCAGCTGTATCTTGTGGAAAACCAGGACC17290.1069056472645386No Hit
GAACAGGACACAGGTCTGTAAGCAACATTTGAACATAGGGGAAGGCTTAG17020.10523621263403395No Hit
GGTCTGTAAGCAACATTTGAACATAGGGGAAGGCTTAGATCGGAAGAGCG16880.10437057986266117No Hit
CCTACACGACGCTCTTCCGATCTCCCTGGGAAATGTCACCTCTTGGCCCC16870.10430874895042026Illumina Single End PCR Primer 1 (100% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16870.10430874895042026No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG16700.10325762344232474No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC16570.10245382158319287No Hit
GCTTACAGACCTGTGTCCTGTTCAGGACCCAGGTATCAGCTGTATCTTGT16290.1007225560404473No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)