FastQCFastQC Report
Sat 10 Sep 2022
EGAF00004920774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1617314
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCTGAACCGCTCTTCCGATCTAAGCCTTCCCCTATGTTCAAATGTTGC185041.1441192001058544Illumina Paired End PCR Primer 2 (100% over 23bp)
CCCTGGGAAATGTCACCTCTTGGCCCCAGAAGATTCCCCAAAGCACAAAA99170.6131771566931344No Hit
TCCCTACACGACGCTCTTCCGATCTCCCTGGGAAATGTCACCTCTTGGCC96460.5964209794758469Illumina Single End PCR Primer 1 (100% over 25bp)
ATCTAAGCCTTCCCCTATGTTCAAATGTTGCTTACAGACCTGTGTCCTGT93070.5754603002261774No Hit
ACGCTCTTCCGATCTCCCTGGGAAATGTCACCTCTTGGCCCCAGAAGATT88060.54448301319348No Hit
GTTCAAATGTTGCTTACAGACCTGTGTCCTGTTCAGGACCCAGGTATCAG86010.5318076761840929No Hit
GTTCAGGACCCAGGTATCAGCTGTATCTTGTGGAAAACCAGGACCATCAT85640.5295199324311791No Hit
CTTCCGATCTCCCTGGGAAATGTCACCTCTTGGCCCCAGAAGATTCCCCA84210.5206781119807286No Hit
GCTGTATCTTGTGGAAAACCAGGACCATCATCTCAGCTTTTGTGCTTTGG77870.48147731361998963No Hit
GGTATCAGCTGTATCTTGTGGAAAACCAGGACCATCATCTCAGCTTTTGT69810.43164159835381377No Hit
TTACAGACCTGTGTCCTGTTCAGGACCCAGGTATCAGCTGTATCTTGTGG67350.4164311939425492No Hit
CTACACGACGCTCTTCCGATCTCCCTGGGAAATGTCACCTCTTGGCCCCA62170.38440278140175627Illumina Single End PCR Primer 1 (100% over 22bp)
TTCCCTACACGACGCTCTTCCGATCTCCCTGGGAAATGTCACCTCTTGGC60840.3761792700737148Illumina Single End PCR Primer 1 (100% over 26bp)
ACACGACGCTCTTCCGATCTCCCTGGGAAATGTCACCTCTTGGCCCCAGA52520.32473595108927517No Hit
AGATACAGCTGATACCTGGGTCCTGAACAGGACACAGGTCTGTAAGCAAC51050.315646806989861No Hit
CCTGTGTCCTGTTCAGGACCCAGGTATCAGCTGTATCTTGTGGAAAACCA50230.31057667218610613No Hit
CCCCAGAAGATTCCCCAAAGCACAAAAGCTGAGATGATGGTCCTGGTTTT39580.24472675064953373No Hit
GGGAAATGTCACCTCTTGGCCCCAGAAGATTCCCCAAAGCACAAAAGCTG37780.23359718644616936No Hit
GTCCTGAACAGGACACAGGTCTGTAAGCAACATTTGAACATAGGGGAAGG36710.22698127883639171No Hit
GATCTAAGCCTTCCCCTATGTTCAAATGTTGCTTACAGACCTGTGTCCTG36250.2241370568733097No Hit
CTTCCGATCTAAGCCTTCCCCTATGTTCAAATGTTGCTTACAGACCTGTG35460.21925241480627755No Hit
GCTGATACCTGGGTCCTGAACAGGACACAGGTCTGTAAGCAACATTTGAA32440.20057947930952183No Hit
TCCGATCTCCCTGGGAAATGTCACCTCTTGGCCCCAGAAGATTCCCCAAA31790.19656047001386248No Hit
CCCCTATGTTCAAATGTTGCTTACAGACCTGTGTCCTGTTCAGGACCCAG30990.19161399703458945No Hit
GTCCTGTTCAGGACCCAGGTATCAGCTGTATCTTGTGGAAAACCAGGACC30460.18833695868582107No Hit
CCCCAAAGCACAAAAGCTGAGATGATGGTCCTGGTTTTCCACAAGATACA29760.18400879482895716No Hit
GACCTGTGTCCTGTTCAGGACCCAGGTATCAGCTGTATCTTGTGGAAAAC28960.17906232184968413No Hit
ATGTTCAAATGTTGCTTACAGACCTGTGTCCTGTTCAGGACCCAGGTATC28090.17368303248472466No Hit
CCGCTCTTCCGATCTAAGCCTTCCCCTATGTTCAAATGTTGCTTACAGAC27550.17034416322371537No Hit
GCACAAAAGCTGAGATGATGGTCCTGGTTTTCCACAAGATACAGCTGATA27260.1685510667687289No Hit
CCTCTTGGCCCCAGAAGATTCCCCAAAGCACAAAAGCTGAGATGATGGTC27190.1681182503830425No Hit
CTCTTGGCCCCAGAAGATTCCCCAAAGCACAAAAGCTGAGATGATGGTCC26270.1624298064568785No Hit
ATACAGCTGATACCTGGGTCCTGAACAGGACACAGGTCTGTAAGCAACAT26240.16224431372015577No Hit
GTCCTGGTTTTCCACAAGATACAGCTGATACCTGGGTCCTGAACAGGACA24650.1524131986738506No Hit
GCCTTCCCCTATGTTCAAATGTTGCTTACAGACCTGTGTCCTGTTCAGGA24610.15216587502488696No Hit
CCCTACACGACGCTCTTCCGATCTCCCTGGGAAATGTCACCTCTTGGCCC23860.14752855660681846Illumina Single End PCR Primer 1 (100% over 24bp)
ATCTTGTGGAAAACCAGGACCATCATCTCAGCTTTTGTGCTTTGGGGAAT21940.13565702145656316No Hit
CTCTTCCGATCTCCCTGGGAAATGTCACCTCTTGGCCCCAGAAGATTCCC21530.13312195405468574No Hit
AACCAGGACCATCATCTCAGCTTTTGTGCTTTGGGGAATCTTCTGGGGCC20440.12638238462042622No Hit
GAACAGGACACAGGTCTGTAAGCAACATTTGAACATAGGGGAAGGCTTAG20350.12582590641025798No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT20340.12576407549801707No Hit
TGTCACCTCTTGGCCCCAGAAGATTCCCCAAAGCACAAAAGCTGAGATGA20260.1252694282000898No Hit
CAGCTGTATCTTGTGGAAAACCAGGACCATCATCTCAGCTTTTGTGCTTT20230.12508393546336705No Hit
GGTCTGTAAGCAACATTTGAACATAGGGGAAGGCTTAGATCGGAAGAGCG20230.12508393546336705No Hit
CTTCTGGGGCCAAGAGGTGACATTTCCCAGGGAGATCGGAAGAGCGTCGT19760.12217788258804413No Hit
GGACACAGGTCTGTAAGCAACATTTGAACATAGGGGAAGGCTTAGATCGG19140.11834436602910753No Hit
GCTTTTGTGCTTTGGGGAATCTTCTGGGGCCAAGAGGTGACATTTCCCAG17340.10721480182574318No Hit
CCTATGTTCAAATGTTGCTTACAGACCTGTGTCCTGTTCAGGACCCAGGT16650.10294846888112019No Hit
GTTGCTTACAGACCTGTGTCCTGTTCAGGACCCAGGTATCAGCTGTATCT16320.10090804877717005No Hit
GTGTCCTGTTCAGGACCCAGGTATCAGCTGTATCTTGTGGAAAACCAGGA16220.10028973965476091No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)