FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13915353
Sequences flagged as poor quality0
Sequence length150
%GC40

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC493780.35484547176057984No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA440710.3167077400048709No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG431910.31038378976084907No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA412180.29620520586146826No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA369730.2656993322411584No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC365690.26279606417458473No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT338370.2431630731897351No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC304550.21885898259282394No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC300680.21607788174687342No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA286970.2062254547189712No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC244070.17539619727936473No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC231620.16644924494549296No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC231450.1663270777248698No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA227010.16313635737447696No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA214260.15397381582774075No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA213750.1536073141658713No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT184420.13252987545483036No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA179200.12877862315099012No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG171270.123079881624275No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA171150.12299364593912925No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC167370.12027722185703806No Hit
GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA157570.11323464090346828No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC154870.11129433798768885No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC140920.10126943958949514No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA482550.059.9487721
GGTATCA293250.049.7068941
AACGCAG625650.045.8165326
ACGCAGA634750.045.2060177
CGCAGAG633600.045.163288
CAACGCA639900.044.8074845
ATCAACG648250.044.1649863
TCAACGC647400.044.1438834
GCAGAGT658100.043.492559
TATCAAC663350.043.2802352
TCTCTGC114800.035.5463838
ACTGTGC125250.035.2813268
CTGTGCA121800.033.857789
ACGTCCA85400.032.6134265
TACGTCC86150.032.2459644
CTCTGCA128050.031.1373279
ATACGTC92100.030.0073363
TTAGGGC199500.028.7161373
AGGGCTA179050.028.2970835
CACTGTG152500.028.2689517
Produced by FastQC (version 0.11.5)