FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6085430
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290570.4774847463531747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120660.19827686786307624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119620.19656786784171373No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA77690.1276655881342814No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA271550.072.452321
GGTATCA110800.064.408931
TCAACGC378000.052.26414
TATCAAC380450.051.8190572
ATCAACG380600.051.6581343
AACGCAG384650.051.402656
CAACGCA386750.051.03435
ACGCAGA405850.048.6586237
CGCAGAG414050.047.7360538
GCAGAGT442300.044.7139859
AGAGTAC428400.034.32890310-11
CAGAGTA433900.030.87474410-11
AGTACGG346450.029.19396412-13
GAGTACG350050.025.94919812-13
GAGTACT166700.025.926512-13
TACGGGC88750.025.59945114-15
TACGGGT81450.024.82695414-15
AGTACTT189950.024.09793512-13
GTACGGG370700.023.43228314-15
TACGGGA133800.023.0255114-15
Produced by FastQC (version 0.11.5)