FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004920891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10005
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.6496751624187905No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.5097451274362819No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.49975012493753124No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG240.23988005997001496No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA230.22988505747126436No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG170.1699150424787606No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT160.15992003998001Clontech Universal Primer Mix Long (96% over 26bp)
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG150.14992503748125938No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA130.12993503248375812Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTT130.12993503248375812No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT120.11994002998500748No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTT120.11994002998500748No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC120.11994002998500748No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTT120.11994002998500748No Hit
GCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTT110.10994502748625688No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT110.10994502748625688No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT110.10994502748625688No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.10994502748625688No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)