FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004920892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10005
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA290.2898550724637681Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG230.22988505747126436No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT190.18990504747626188No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA170.1699150424787606No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT130.12993503248375812No Hit
GGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG120.11994002998500748No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC110.10994502748625688No Hit
GCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTT110.10994502748625688No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)